3 end Search Results


endtag dna end labeling 33 kit  (Vector Laboratories)
92
Vector Laboratories endtag dna end labeling 33 kit
Endtag Dna End Labeling 33 Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bio 3 end oligonucleotide labelling kit  (Jena Bioscience)
90
Jena Bioscience bio 3 end oligonucleotide labelling kit
Bio 3 End Oligonucleotide Labelling Kit, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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protease inhibitor mixture  (Boster Bio)
94
Boster Bio protease inhibitor mixture
Protease Inhibitor Mixture, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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eb3  (Proteintech)
93
Proteintech eb3
Characterization of cell lines with disrupted EB-encoding genes. (A) Schemes of EB1, EB2, and <t>EB3</t> proteins and the position of gRNA sequences. Arrow indicates the in-frame Met at the beginning of the EB2 CH domain. (B and C) Western blot analysis and immunostaining of control and the EB mutant cells with the indicated antibodies. (D and E) Quantification of the mitotic index and mitotic stages in the indicated cell lines. (D) n ≥ 3 and (E) n = 4 experiments, with 3,000 cells each. (F) Mean intensity of EB2 and α-tubulin staining starting from the MT tip (0); 26–28 MT ends analyzed per condition from 6–10 cells. *, P < 0.05; **, P < 0.01 (Mann-Whitney U test).
Eb3, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/eb3/product/Proteintech
Average 93 stars, based on 1 article reviews
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dig oligonucleotide 3p-end labeling kit  (Boehringer Mannheim)
90
Boehringer Mannheim dig oligonucleotide 3p-end labeling kit
Characterization of cell lines with disrupted EB-encoding genes. (A) Schemes of EB1, EB2, and <t>EB3</t> proteins and the position of gRNA sequences. Arrow indicates the in-frame Met at the beginning of the EB2 CH domain. (B and C) Western blot analysis and immunostaining of control and the EB mutant cells with the indicated antibodies. (D and E) Quantification of the mitotic index and mitotic stages in the indicated cell lines. (D) n ≥ 3 and (E) n = 4 experiments, with 3,000 cells each. (F) Mean intensity of EB2 and α-tubulin staining starting from the MT tip (0); 26–28 MT ends analyzed per condition from 6–10 cells. *, P < 0.05; **, P < 0.01 (Mann-Whitney U test).
Dig Oligonucleotide 3p End Labeling Kit, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dig oligonucleotide 3p-end labeling kit/product/Boehringer Mannheim
Average 90 stars, based on 1 article reviews
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tunel dna 3-end labeling kit  (Oncor Inc)
90
Oncor Inc tunel dna 3-end labeling kit
Characterization of cell lines with disrupted EB-encoding genes. (A) Schemes of EB1, EB2, and <t>EB3</t> proteins and the position of gRNA sequences. Arrow indicates the in-frame Met at the beginning of the EB2 CH domain. (B and C) Western blot analysis and immunostaining of control and the EB mutant cells with the indicated antibodies. (D and E) Quantification of the mitotic index and mitotic stages in the indicated cell lines. (D) n ≥ 3 and (E) n = 4 experiments, with 3,000 cells each. (F) Mean intensity of EB2 and α-tubulin staining starting from the MT tip (0); 26–28 MT ends analyzed per condition from 6–10 cells. *, P < 0.05; **, P < 0.01 (Mann-Whitney U test).
Tunel Dna 3 End Labeling Kit, supplied by Oncor Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tunel dna 3-end labeling kit/product/Oncor Inc
Average 90 stars, based on 1 article reviews
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oligo(dt) primer labeled with digoxigenin at the 3' end  (Biomer Technology Ltd)
90
Biomer Technology Ltd oligo(dt) primer labeled with digoxigenin at the 3' end
Characterization of cell lines with disrupted EB-encoding genes. (A) Schemes of EB1, EB2, and <t>EB3</t> proteins and the position of gRNA sequences. Arrow indicates the in-frame Met at the beginning of the EB2 CH domain. (B and C) Western blot analysis and immunostaining of control and the EB mutant cells with the indicated antibodies. (D and E) Quantification of the mitotic index and mitotic stages in the indicated cell lines. (D) n ≥ 3 and (E) n = 4 experiments, with 3,000 cells each. (F) Mean intensity of EB2 and α-tubulin staining starting from the MT tip (0); 26–28 MT ends analyzed per condition from 6–10 cells. *, P < 0.05; **, P < 0.01 (Mann-Whitney U test).
Oligo(Dt) Primer Labeled With Digoxigenin At The 3' End, supplied by Biomer Technology Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/oligo(dt) primer labeled with digoxigenin at the 3' end/product/Biomer Technology Ltd
Average 90 stars, based on 1 article reviews
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oligonucleotide 5′-tatttctgatgtccaccccc-3′  (Generi Biotech)
90
Generi Biotech oligonucleotide 5′-tatttctgatgtccaccccc-3′
Characterization of cell lines with disrupted EB-encoding genes. (A) Schemes of EB1, EB2, and <t>EB3</t> proteins and the position of gRNA sequences. Arrow indicates the in-frame Met at the beginning of the EB2 CH domain. (B and C) Western blot analysis and immunostaining of control and the EB mutant cells with the indicated antibodies. (D and E) Quantification of the mitotic index and mitotic stages in the indicated cell lines. (D) n ≥ 3 and (E) n = 4 experiments, with 3,000 cells each. (F) Mean intensity of EB2 and α-tubulin staining starting from the MT tip (0); 26–28 MT ends analyzed per condition from 6–10 cells. *, P < 0.05; **, P < 0.01 (Mann-Whitney U test).
Oligonucleotide 5′ Tatttctgatgtccaccccc 3′, supplied by Generi Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/oligonucleotide 5′-tatttctgatgtccaccccc-3′/product/Generi Biotech
Average 90 stars, based on 1 article reviews
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biotin 3'-end dna labeling kit  (Fisher Scientific)
90
Fisher Scientific biotin 3'-end dna labeling kit
Characterization of cell lines with disrupted EB-encoding genes. (A) Schemes of EB1, EB2, and <t>EB3</t> proteins and the position of gRNA sequences. Arrow indicates the in-frame Met at the beginning of the EB2 CH domain. (B and C) Western blot analysis and immunostaining of control and the EB mutant cells with the indicated antibodies. (D and E) Quantification of the mitotic index and mitotic stages in the indicated cell lines. (D) n ≥ 3 and (E) n = 4 experiments, with 3,000 cells each. (F) Mean intensity of EB2 and α-tubulin staining starting from the MT tip (0); 26–28 MT ends analyzed per condition from 6–10 cells. *, P < 0.05; **, P < 0.01 (Mann-Whitney U test).
Biotin 3' End Dna Labeling Kit, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotin 3'-end dna labeling kit/product/Fisher Scientific
Average 90 stars, based on 1 article reviews
biotin 3'-end dna labeling kit - by Bioz Stars, 2026-04
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biotin 3' 0 end dna labeling kit d3106  (Beyotime)
90
Beyotime biotin 3' 0 end dna labeling kit d3106
Characterization of cell lines with disrupted EB-encoding genes. (A) Schemes of EB1, EB2, and <t>EB3</t> proteins and the position of gRNA sequences. Arrow indicates the in-frame Met at the beginning of the EB2 CH domain. (B and C) Western blot analysis and immunostaining of control and the EB mutant cells with the indicated antibodies. (D and E) Quantification of the mitotic index and mitotic stages in the indicated cell lines. (D) n ≥ 3 and (E) n = 4 experiments, with 3,000 cells each. (F) Mean intensity of EB2 and α-tubulin staining starting from the MT tip (0); 26–28 MT ends analyzed per condition from 6–10 cells. *, P < 0.05; **, P < 0.01 (Mann-Whitney U test).
Biotin 3' 0 End Dna Labeling Kit D3106, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotin 3' 0 end dna labeling kit d3106/product/Beyotime
Average 90 stars, based on 1 article reviews
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16s rrna gene sequencing 2 3 150 paired-end sequencing  (Illumina Inc)
90
Illumina Inc 16s rrna gene sequencing 2 3 150 paired-end sequencing
Characterization of cell lines with disrupted EB-encoding genes. (A) Schemes of EB1, EB2, and <t>EB3</t> proteins and the position of gRNA sequences. Arrow indicates the in-frame Met at the beginning of the EB2 CH domain. (B and C) Western blot analysis and immunostaining of control and the EB mutant cells with the indicated antibodies. (D and E) Quantification of the mitotic index and mitotic stages in the indicated cell lines. (D) n ≥ 3 and (E) n = 4 experiments, with 3,000 cells each. (F) Mean intensity of EB2 and α-tubulin staining starting from the MT tip (0); 26–28 MT ends analyzed per condition from 6–10 cells. *, P < 0.05; **, P < 0.01 (Mann-Whitney U test).
16s Rrna Gene Sequencing 2 3 150 Paired End Sequencing, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/16s rrna gene sequencing 2 3 150 paired-end sequencing/product/Illumina Inc
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3′ end biotinylated rna oligonucleotides pts6  (LGC Biosearch)
90
LGC Biosearch 3′ end biotinylated rna oligonucleotides pts6
Characterization of cell lines with disrupted EB-encoding genes. (A) Schemes of EB1, EB2, and <t>EB3</t> proteins and the position of gRNA sequences. Arrow indicates the in-frame Met at the beginning of the EB2 CH domain. (B and C) Western blot analysis and immunostaining of control and the EB mutant cells with the indicated antibodies. (D and E) Quantification of the mitotic index and mitotic stages in the indicated cell lines. (D) n ≥ 3 and (E) n = 4 experiments, with 3,000 cells each. (F) Mean intensity of EB2 and α-tubulin staining starting from the MT tip (0); 26–28 MT ends analyzed per condition from 6–10 cells. *, P < 0.05; **, P < 0.01 (Mann-Whitney U test).
3′ End Biotinylated Rna Oligonucleotides Pts6, supplied by LGC Biosearch, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Characterization of cell lines with disrupted EB-encoding genes. (A) Schemes of EB1, EB2, and EB3 proteins and the position of gRNA sequences. Arrow indicates the in-frame Met at the beginning of the EB2 CH domain. (B and C) Western blot analysis and immunostaining of control and the EB mutant cells with the indicated antibodies. (D and E) Quantification of the mitotic index and mitotic stages in the indicated cell lines. (D) n ≥ 3 and (E) n = 4 experiments, with 3,000 cells each. (F) Mean intensity of EB2 and α-tubulin staining starting from the MT tip (0); 26–28 MT ends analyzed per condition from 6–10 cells. *, P < 0.05; **, P < 0.01 (Mann-Whitney U test).

Journal: The Journal of Cell Biology

Article Title: EB1 and EB3 regulate microtubule minus end organization and Golgi morphology

doi: 10.1083/jcb.201701024

Figure Lengend Snippet: Characterization of cell lines with disrupted EB-encoding genes. (A) Schemes of EB1, EB2, and EB3 proteins and the position of gRNA sequences. Arrow indicates the in-frame Met at the beginning of the EB2 CH domain. (B and C) Western blot analysis and immunostaining of control and the EB mutant cells with the indicated antibodies. (D and E) Quantification of the mitotic index and mitotic stages in the indicated cell lines. (D) n ≥ 3 and (E) n = 4 experiments, with 3,000 cells each. (F) Mean intensity of EB2 and α-tubulin staining starting from the MT tip (0); 26–28 MT ends analyzed per condition from 6–10 cells. *, P < 0.05; **, P < 0.01 (Mann-Whitney U test).

Article Snippet: We used rabbit antibodies against CAMSAP2 (NBP1-21402, Novus; 17880-1-AP, Proteintech), EB3 , CLIP170 and CLASP1 , GM130 (ab52649; Abcam), GFP (ab290; Abcam), CEP135 (SAB4503685-100UG; Sigma-Aldrich), and goat anti–myosin IIB antibody (sc-47205; Santa Cruz).

Techniques: Western Blot, Immunostaining, Control, Mutagenesis, Staining, MANN-WHITNEY

EB interaction with MMG is required to recruit CAMSAP2 stretches to the Golgi. (A and D) Immunostaining for CAMSAP2 and GM130 of EB1/3mut (A) or MMG knockout (D) RPE1 cells expressing the indicated constructs. (B and F) Percentage of cells with CAMSAP2 stretches in the Golgi area in EB1/3mut (B) or MMG knockout (F) RPE1 cells expressing the indicated constructs. n = 77–112 transfected cells in B and 41–81 transfected cells in F. **, P < 0.01; ***, P < 0.001 (Student’s t test). (C) A scheme of MMG–EB3 interaction and the fusion constructs used. (E and G) Streptavidin pull-down assays with the extracts of HEK293T cells coexpressing the indicated biotinylation tag (Bio)-GFP–MMG fusions, the indicated EB–GFP fusions, and biotin ligase BirA, analyzed by Western blotting with anti-GFP antibodies.

Journal: The Journal of Cell Biology

Article Title: EB1 and EB3 regulate microtubule minus end organization and Golgi morphology

doi: 10.1083/jcb.201701024

Figure Lengend Snippet: EB interaction with MMG is required to recruit CAMSAP2 stretches to the Golgi. (A and D) Immunostaining for CAMSAP2 and GM130 of EB1/3mut (A) or MMG knockout (D) RPE1 cells expressing the indicated constructs. (B and F) Percentage of cells with CAMSAP2 stretches in the Golgi area in EB1/3mut (B) or MMG knockout (F) RPE1 cells expressing the indicated constructs. n = 77–112 transfected cells in B and 41–81 transfected cells in F. **, P < 0.01; ***, P < 0.001 (Student’s t test). (C) A scheme of MMG–EB3 interaction and the fusion constructs used. (E and G) Streptavidin pull-down assays with the extracts of HEK293T cells coexpressing the indicated biotinylation tag (Bio)-GFP–MMG fusions, the indicated EB–GFP fusions, and biotin ligase BirA, analyzed by Western blotting with anti-GFP antibodies.

Article Snippet: We used rabbit antibodies against CAMSAP2 (NBP1-21402, Novus; 17880-1-AP, Proteintech), EB3 , CLIP170 and CLASP1 , GM130 (ab52649; Abcam), GFP (ab290; Abcam), CEP135 (SAB4503685-100UG; Sigma-Aldrich), and goat anti–myosin IIB antibody (sc-47205; Santa Cruz).

Techniques: Immunostaining, Knock-Out, Expressing, Construct, Transfection, Western Blot

Effect of EB1, EB3, and CAMSAP2 disruption on Golgi organization and MT nucleation from Golgi. (A) Western blots of the extracts of EB1/3/CAMSAP2mut RPE1 cells. (B and C) Immunostaining for α-tubulin and GM130 (B) or γ-tubulin (C) in EB1/3/CAMSAP2mut RPE1 cells. In C, enlarged images of the boxed areas are shown below. (D and E) Immunostaining for GM130 and quantification of the Golgi area in the indicated cell lines. n = 50 cells per condition. (F) Immunostaining of GM130 and α-tubulin in the indicated cell lines after a 3-min recovery from nocodazole treatment. (G) Tubulin intensity in the vicinity of Golgi membranes in the indicated cell lines treated as in F. n = 30 cells per condition. n.s., no significant differences; **, P < 0.01; ***, P < 0.001 (Mann-Whitney U test).

Journal: The Journal of Cell Biology

Article Title: EB1 and EB3 regulate microtubule minus end organization and Golgi morphology

doi: 10.1083/jcb.201701024

Figure Lengend Snippet: Effect of EB1, EB3, and CAMSAP2 disruption on Golgi organization and MT nucleation from Golgi. (A) Western blots of the extracts of EB1/3/CAMSAP2mut RPE1 cells. (B and C) Immunostaining for α-tubulin and GM130 (B) or γ-tubulin (C) in EB1/3/CAMSAP2mut RPE1 cells. In C, enlarged images of the boxed areas are shown below. (D and E) Immunostaining for GM130 and quantification of the Golgi area in the indicated cell lines. n = 50 cells per condition. (F) Immunostaining of GM130 and α-tubulin in the indicated cell lines after a 3-min recovery from nocodazole treatment. (G) Tubulin intensity in the vicinity of Golgi membranes in the indicated cell lines treated as in F. n = 30 cells per condition. n.s., no significant differences; **, P < 0.01; ***, P < 0.001 (Mann-Whitney U test).

Article Snippet: We used rabbit antibodies against CAMSAP2 (NBP1-21402, Novus; 17880-1-AP, Proteintech), EB3 , CLIP170 and CLASP1 , GM130 (ab52649; Abcam), GFP (ab290; Abcam), CEP135 (SAB4503685-100UG; Sigma-Aldrich), and goat anti–myosin IIB antibody (sc-47205; Santa Cruz).

Techniques: Disruption, Western Blot, Immunostaining, MANN-WHITNEY

Effect of EB1, EB3, and CAMSAP2 disruption on MT and Golgi organization after centriole depletion. (A and B) Immunostaining for CEP135 and γ-tubulin (A) and percentage of γ-tubulin– and CEP135-positive cells in the indicated cell lines after 11 d of treatment with 125 nM centrinone (B). n = 366–391 cells per condition. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (Student’s t test). (C and D) Immunostaining for α-tubulin (C) or CAMSAP2 (D) and GM130 in the indicated cell lines treated with centrinone. Enlarged portions of the boxed areas are shown at the bottom. (E and F) Percentage of cells with CAMSAP2 stretches at the Golgi (E) and quantification of the CAMSAP2 stretch length (F) for the indicated cell lines and treatments. n = 248–418 cells per condition in E and 199–260 CAMSAPP2 stretches in F. **, P < 0.01; ***, P < 0.001 (Mann-Whitney U test). (G) Quantification of Golgi morphology for the indicated PRE1 cell lines and treatments. Three types of Golgi organization are shown on the left. 335–642 control and 153–593 centrinone-treated cells were analyzed. Significant differences between values are indicated: *, control (DMSO) treatment; #, centrinone treatment. * or #, P < 0.05; ** or ##, P < 0.01 (Mann-Whitney U test).

Journal: The Journal of Cell Biology

Article Title: EB1 and EB3 regulate microtubule minus end organization and Golgi morphology

doi: 10.1083/jcb.201701024

Figure Lengend Snippet: Effect of EB1, EB3, and CAMSAP2 disruption on MT and Golgi organization after centriole depletion. (A and B) Immunostaining for CEP135 and γ-tubulin (A) and percentage of γ-tubulin– and CEP135-positive cells in the indicated cell lines after 11 d of treatment with 125 nM centrinone (B). n = 366–391 cells per condition. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (Student’s t test). (C and D) Immunostaining for α-tubulin (C) or CAMSAP2 (D) and GM130 in the indicated cell lines treated with centrinone. Enlarged portions of the boxed areas are shown at the bottom. (E and F) Percentage of cells with CAMSAP2 stretches at the Golgi (E) and quantification of the CAMSAP2 stretch length (F) for the indicated cell lines and treatments. n = 248–418 cells per condition in E and 199–260 CAMSAPP2 stretches in F. **, P < 0.01; ***, P < 0.001 (Mann-Whitney U test). (G) Quantification of Golgi morphology for the indicated PRE1 cell lines and treatments. Three types of Golgi organization are shown on the left. 335–642 control and 153–593 centrinone-treated cells were analyzed. Significant differences between values are indicated: *, control (DMSO) treatment; #, centrinone treatment. * or #, P < 0.05; ** or ##, P < 0.01 (Mann-Whitney U test).

Article Snippet: We used rabbit antibodies against CAMSAP2 (NBP1-21402, Novus; 17880-1-AP, Proteintech), EB3 , CLIP170 and CLASP1 , GM130 (ab52649; Abcam), GFP (ab290; Abcam), CEP135 (SAB4503685-100UG; Sigma-Aldrich), and goat anti–myosin IIB antibody (sc-47205; Santa Cruz).

Techniques: Disruption, Immunostaining, MANN-WHITNEY, Control

Effects of EB1 and EB3 disruption on cell migration. (A) Phase-contrast images of monolayer wound-healing assays in control and EB1/3mut RPE1 cells at the indicated time points and percentage of wound area closure. n = 3 independent experiments. (B) Immunostaining for α-tubulin and GM130 in a wound healing assay and the quantification of the Golgi reorientation. n = 163 control and 152 EB1/3mut RPE1 cells. (C). Phase contrast images and tracks of control and EB1/3mut cells during 7-h migration in sparse culture (C). (D) Quantification of cell migration velocity in control and EB1/3mut RPE1 cells expressing the indicated constructs. n = 30–60 cells. (E–H) Immunostaining for paxillin in the indicated cell lines (E), quantification of FA size in the whole cell (F), and FA size (G) and number (H) in the inner cell area (with 5-µm-broad cell rim excluded, red dotted lines in E). n = 44 control and 55 EB1/3mut RPE1 cells; per condition, 3,000 and 1,000 FAs were analyzed in the whole cell and the inner cell area, respectively. (I and J) Phase contrast images and tracks of control and EB1/3mut HT1080 cells in 3D matrix during 24-h migration (I) and quantification of their migration velocity (J). n = 24 control and 26 EB1/3mut HT1080 cells. (K and L) Morphology of control and EB1/3mut HT1080 cells in 3D matrix and the mean number of protrusions in these cells. n = 65 control and 63 EB1/3mut HT1080 cells. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (Mann-Whitney U test).

Journal: The Journal of Cell Biology

Article Title: EB1 and EB3 regulate microtubule minus end organization and Golgi morphology

doi: 10.1083/jcb.201701024

Figure Lengend Snippet: Effects of EB1 and EB3 disruption on cell migration. (A) Phase-contrast images of monolayer wound-healing assays in control and EB1/3mut RPE1 cells at the indicated time points and percentage of wound area closure. n = 3 independent experiments. (B) Immunostaining for α-tubulin and GM130 in a wound healing assay and the quantification of the Golgi reorientation. n = 163 control and 152 EB1/3mut RPE1 cells. (C). Phase contrast images and tracks of control and EB1/3mut cells during 7-h migration in sparse culture (C). (D) Quantification of cell migration velocity in control and EB1/3mut RPE1 cells expressing the indicated constructs. n = 30–60 cells. (E–H) Immunostaining for paxillin in the indicated cell lines (E), quantification of FA size in the whole cell (F), and FA size (G) and number (H) in the inner cell area (with 5-µm-broad cell rim excluded, red dotted lines in E). n = 44 control and 55 EB1/3mut RPE1 cells; per condition, 3,000 and 1,000 FAs were analyzed in the whole cell and the inner cell area, respectively. (I and J) Phase contrast images and tracks of control and EB1/3mut HT1080 cells in 3D matrix during 24-h migration (I) and quantification of their migration velocity (J). n = 24 control and 26 EB1/3mut HT1080 cells. (K and L) Morphology of control and EB1/3mut HT1080 cells in 3D matrix and the mean number of protrusions in these cells. n = 65 control and 63 EB1/3mut HT1080 cells. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (Mann-Whitney U test).

Article Snippet: We used rabbit antibodies against CAMSAP2 (NBP1-21402, Novus; 17880-1-AP, Proteintech), EB3 , CLIP170 and CLASP1 , GM130 (ab52649; Abcam), GFP (ab290; Abcam), CEP135 (SAB4503685-100UG; Sigma-Aldrich), and goat anti–myosin IIB antibody (sc-47205; Santa Cruz).

Techniques: Disruption, Migration, Control, Immunostaining, Wound Healing Assay, Expressing, Construct, MANN-WHITNEY

Model for EB-dependent Golgi–MT coorganization. MMG associates with Golgi membranes through AKAP450 and GM130 and recruits EB1 and EB3, which bind to MT shafts. CAMSAP2 forms MT minus end-localized stretches, which associate with Golgi membranes by binding to the complex of AKAP450 and MMG. EB1 and EB3 also regulate the length of CAMSAP2-decorated MT stretches, likely by controlling MT end dynamics together with some of their partners such as CLASPs. Association of Golgi membranes with free, CAMSAP2-decorated MT minus ends can promote their clustering through dynein-driven minus end–directed transport. Golgi-associated EB1 and EB3 promote spreading of Golgi ribbons along MTs and counteract their dynein-mediated compaction (double-headed arrows).

Journal: The Journal of Cell Biology

Article Title: EB1 and EB3 regulate microtubule minus end organization and Golgi morphology

doi: 10.1083/jcb.201701024

Figure Lengend Snippet: Model for EB-dependent Golgi–MT coorganization. MMG associates with Golgi membranes through AKAP450 and GM130 and recruits EB1 and EB3, which bind to MT shafts. CAMSAP2 forms MT minus end-localized stretches, which associate with Golgi membranes by binding to the complex of AKAP450 and MMG. EB1 and EB3 also regulate the length of CAMSAP2-decorated MT stretches, likely by controlling MT end dynamics together with some of their partners such as CLASPs. Association of Golgi membranes with free, CAMSAP2-decorated MT minus ends can promote their clustering through dynein-driven minus end–directed transport. Golgi-associated EB1 and EB3 promote spreading of Golgi ribbons along MTs and counteract their dynein-mediated compaction (double-headed arrows).

Article Snippet: We used rabbit antibodies against CAMSAP2 (NBP1-21402, Novus; 17880-1-AP, Proteintech), EB3 , CLIP170 and CLASP1 , GM130 (ab52649; Abcam), GFP (ab290; Abcam), CEP135 (SAB4503685-100UG; Sigma-Aldrich), and goat anti–myosin IIB antibody (sc-47205; Santa Cruz).

Techniques: Binding Assay

Primers used to generate the DNA constructs

Journal: The Journal of Cell Biology

Article Title: EB1 and EB3 regulate microtubule minus end organization and Golgi morphology

doi: 10.1083/jcb.201701024

Figure Lengend Snippet: Primers used to generate the DNA constructs

Article Snippet: We used rabbit antibodies against CAMSAP2 (NBP1-21402, Novus; 17880-1-AP, Proteintech), EB3 , CLIP170 and CLASP1 , GM130 (ab52649; Abcam), GFP (ab290; Abcam), CEP135 (SAB4503685-100UG; Sigma-Aldrich), and goat anti–myosin IIB antibody (sc-47205; Santa Cruz).

Techniques: Sequencing